Antimicrobial properties of dentine bonding agents determined using in vitro and ex vivo methods
A guide to the potential antimicrobial activity of a DBA may be gained from an assessment of its pH when added to bacteriological culture medium. The direct contact method gives a better reflection of the killing of bacteria in infected dentine than the disk diffusion method. Killing in the ex vivo model gives a more realistic and more reliable method for determining the antibacterial activity of a given DBA and that comparisons of the relative inhibitory activity of DBAs should be tested using this ex vivo model.
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